Regarding how to adjust the mobile phase in the development of HPLC analysis methods, if you have many years of practical experience in chromatography, you will find three secrets. Let us share with you the technical engineers of Hangzhou Celtech Technology Co., Ltd.
Tip 1: From strong to weak: generally use 90% acetonitrile (or methanol) / water (or buffer solution) for the test, so that the separation results can be obtained quickly, and then adjust the organic solvent (acetonitrile or methanol) according to the peak condition )proportion.
Tip 2: The triple rule: For every 10% reduction in the amount of organic solvent (methanol or acetonitrile), the retention factor increases about three times. This is a triple rule. This is a smart and labor-saving method. During the adjustment, pay attention to observe the separation of each peak.
Tip 3: Coarse adjustment to fine adjustment: When the separation reaches a certain level, the organic solvent 10% change should be adjusted to 5%, and the adjustment rate should be gradually reduced according to this rule until the separation of each component no longer changes.
First 100% organic solvent, and then down. Because some separation can be separated by 96%
If you want to have any other chromatographic knowledge and want to communicate together, you can contact our technical engineers.
Related products: syltech 500 high performance liquid chromatograph (single pump), VERTEX STI 5000 isocratic (single pump) system standard configuration, N2000 chromatography data workstation (SP1 version)
Consultation hotline for liquid chromatograph; please download and reference for more instrument information.
Hangzhou Celtech Technology Co., Ltd. 2009-02
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