Research Background:
The current stem cell culture of mouse bone marrow mesenchymal stem cells is difficult. The bone marrow stromal cells of mice have complex components and low ratio of mesenchymal stem cells, which is difficult to isolate and culture. Dr. Chen, a doctoral student at the Guangdong Medical College, encountered this problem during the training process. He has successfully raised adult and rat bone marrow mesenchymal stem cells. However, the newly cultured mouse mesenchymal stem cells are in poor condition. According to my own analysis: there is no problem with the cell culture operation, the culture conditions are also good, and the reagent source is reliable. The problem should appear on the media formulation. Based on the original bone marrow mesenchymal stem cell culture medium, Dr. Chen modified the formula according to the tutor's point of view, but the cells were not bad. To this end, he did two orthogonalities, and the results were not ideal, and the cell state was unstable. Dr. Chen was so worried that he could not proceed with the next experiment. After the introduction of friends, I found the technical support of Bai Enwei.
solution:
Cultivation techniques, materials and reagent sources, etc., do not feel that there is a big problem; the main reason for the problem is the distribution ratio of the medium. It is recommended that Dr. Chen reconstitute the medium or use ready-made complete medium.
Selected product: mouse mesenchymal stem cell culture medium
Implementation steps:
1. Using the enzymatic mesenchymal stem cell culture medium of Bainwei, the original materials were taken;
2. At the same time, the MSC cells in the medium of Bainwei were significantly better than the culture medium configured by themselves;
3. After passage, the state of the cells and the ability to proliferate are very good;
4. Successfully obtained the mouse MSC needed for the next experiment.
Analysis of the results: the cells are in good condition, forming a fusiform, tightly distributed, and uniform size. It has been passed to 20 generations. Dr. Chen felt a lot about the detour. In order to complete the project experiment efficiently, it is necessary to change the concept, rationally utilize and integrate the existing advantageous resources, and quickly reach the experimental goal.
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