Basic working principle and main use of UV spectrophotometer

The basic working principle of the UV spectrophotometer:

The basic working principle of the ultraviolet spectrophotometer is similar to that of the infrared spectrometer. The organic matter analyzed by a certain frequency of ultraviolet visible light is irradiated, causing a transition of the valence electrons in the molecule, which will be selectively absorbed. A set of spectra that absorbs as a function of wavelength reflects the characteristics of the sample. In the ultraviolet-visible range, for a specific wavelength, the degree of absorption is proportional to the concentration of the component in the sample, so the measured spectrum can be qualitatively analyzed and can be performed based on the comparison of absorption with a known concentration of the standard. Quantitative analysis.

The main purpose of the ultraviolet spectrophotometer:

UV-visible spectrometers cover a wavelength range of 0.2-0.8 microns (corresponding to a wave number of 50,000-12500 cm-1), which is widely used in organic chemistry research. It is commonly used as a material identification, purity check, and study of organic molecular structures. In terms of quantification, the content of each component in a relatively complex structure and mixture can be determined, and the dissociation constant of the substance, the stability constant of the complex, the molecular weight identification of the substance and the indicated endpoint in the microtiter and the high performance liquid chromatography can be determined. Used as a detector, etc.

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