This reagent is for research use only: This kit is used to determine the testosterone (T) content in rat serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the testosterone (T) level in the specimen. The microporous plate was coated with purified rat testosterone (T) antibody to prepare a solid phase antibody, and testosterone (T) was sequentially added to the microcapsules of the coated monoclonal antibody, and then combined with HRP-labeled testosterone (T) antibody to form The antibody-antigen-enzyme-labeled antibody complex was thoroughly washed and added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with testosterone (T) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat testosterone (T) in the sample was calculated from a standard curve. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 part 1 part sealing film 2 pieces (48) 2 pieces (96) Sealed bag 1 1 enzyme label coated plate 1 × 48 1 × 96 2 -8 ° C preservation standard: 180 nmol / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ° C preservation enzyme standard reagent 3 ml ×1 bottle 6 ml×1 bottle 2-8°C Preservation sample dilution 3 ml×1 bottle 6 ml×1 bottle 2-8°C Preservation developer A solution 3 ml×1 bottle 6 ml×1 bottle 2-8°C Preservation developer B solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml ×30 times) × 1 bottle 2-8 ° C preservation
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