The ultra-clean workbench is a facility that provides a sterile operating environment for laboratory work to protect the experiment from the external environment, and at the same time provides a certain degree of protection for the external environment to prevent pollution and protect the operator. Compared with the simple sterile hood, the ultra-clean table has the advantages of allowing the operator to move freely, easily reach any place in the operation area, and have a higher totality.
(1) Principle The clean environment of the ultra-clean workbench is formed in a specific space where clean air (inlet air) flows in a set direction. According to the airflow direction, the existing ultra-clean workbench can be divided into vertical type, inside-out type and side type. Considering the operation quality and the impact on the environment, the vertical type is superior. The clean air provided by the air supply filter plate descends through the operation area at a specific speed, is divided at about the middle of the operation area, is sucked away by the front air suction hole and the rear suction window, and the air sucked at the front and rear of the lower part of the operation area is mixed Together, and pumped into the back positive pressure area by the blower, in the upper part of the machine, 30% of the gas is discharged from the top through the exhaust filter plate, and about 70% of the gas re-enters the operation area through the oxygen supply filter plate. To supplement the air exhausted from the exhaust port, air of the same volume is replenished from the room air through the operating port. This air will never enter the operation area, but just form an air barrier.
(2) The ultra-clean workbench where the debugging is located has undergone rigorous testing before leaving the factory to ensure normal use. But this does not mean that the manufacturer's test can replace the necessary inspection and debugging before the operator uses it. Because the environment of the ultra-clean workbench is different, only after proper inspection and debugging can the role of the equipment be maximized. Before commissioning, a better environment should be selected for the machine. It is best to put it in a sterile room with air disinfection facilities. If the conditions are not available, the machine should be placed in a clean room with few people walking. Adjust the height of each foot to ensure stability and the level of the operating surface. The power supply of the ultra-clean workbench should use a special circuit to avoid the change of air flow rate caused by circuit overload. Compared with the simple sterile hood, the ultra-clean table has the standard configuration of the ultra-clean workbench, which allows the operator to move freely, easily reach any place in the operation area, and the ultraviolet sterilization lamp and lighting fluorescent lamp. The blower provides the power for air flow It is clear at a glance whether these parts are working properly. The most difficult and most important thing is to check the air filter plate and its tightness, which is directly related to the normal use of the machine. The simplest test method is the nutrient agar plate method. Newly-purchased and unused ultra-clean workbenches should be fumigated in addition to being irradiated with ultraviolet lamps, etc., and then place an open nutrient agar plate at the four corners and the center of the operation area when the machine is in working condition After two hours, cover and place in a 37 ° C incubator for 24 hours to calculate the number of colonies. The average number of colonies per plate must be less than 0.5.
(3) Before using the ultra-clean workbench, apply ultraviolet light for 30-40 minutes, and check that various openable doors and windows around the operation area are in working position. The operation is best carried out in the center of the operation area, which is a safer area by design. Before the operation, you should have a preliminary understanding of the experimental materials, and at the same time understand the performance and safety level of the equipment you use. Strictly implement laboratory safety regulations. The use of specific pathogens in any ultra-clean workbench must be evaluated for safety. If the experimental material will cause environmental pollution to the surrounding environment, it should be avoided in models without exhaust filter plates, because operation in flowing air is no different from poisoning. Any advanced equipment does not guarantee the success of the experiment. The use of the ultra-clean workbench in the animal quarantine laboratory is for the purpose of sterility and avoiding cross-contamination. Therefore, skilled operation and clear sterility methods are essential.
(4) Maintenance The ultra-clean workbench is a more precise electrical equipment, and it is very important to carry out regular maintenance and maintenance on it. First, keep the room dry and clean. Moist air will not only rust the manufacturing materials, but also affect the normal operation of electrical circuits. Moist air also facilitates the growth of bacteria and mold. The clean environment can also extend the service life of the filter plate. In addition, regular cleaning of the equipment is an important part of normal use. Cleaning should include routine cleaning before and after use and regular treatment. During fumigation, all gaps should be completely sealed. If the operation port is equipped with a movable baffle type ultra-clean workbench, it can be sealed with plastic film. The filter plates and UV germicidal lamps of the ultra-clean workbench have a calibrated service life and should be replaced on schedule.
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