Primary cell cryopreservation technology

Cell 1. Choose primary cells with good growth conditions and a large number, and change the medium once a day before freezing. Cell 2. Adherent cells need to be digested with 0.25% trypsin to digest the cells, and the cell suspension is collected into a centrifuge tube. The cells were centrifuged at 3, 1000 rpm for 5 minutes, and the supernatant was discarded. 4. Cells, pellets plus DMSO-containing culture solution, count and adjust to (1-10) × 106 / ml. Cell 5. Divide the suspension into cryovials, 1 ml per tube. Cell 6. Seal the cryopreservation tube, the seal must be strict, otherwise it will easily burst during recovery. Cell 7. Mark the cell type with a marker and freeze the date. Cell 8. Cool down in the following order: room temperature → 4 ℃ (20 minutes) → refrigerator freezer (30 minutes) → ultra-low temperature refrigerator (-80 ℃ overnight) → liquid nitrogen.

Descriptions for the series products:

1. The process of all the boards and walls of the series products are completed in one time by large machining center which can ensure the precision of all stations and the equipment operates more steadily.

2. The paper advance mechanism of facial tissue: We can ensure the paper feeding is in high speed and steady whether for thick paper or thin paper for the application of high speed dedicated Feeding Head.

3. Elevating stage of facial tissue: In line with the high speed FeiDa, We adopt high strength turbine reducer which can ensure the stability of accelerating instantly.

4. The paper supporter of facial tissue: We adopt the unique designation of E-type double gap which can push the produced paper directly with hand fork lifter, shorten the time of stacking paper and improve efficiency.

5. Body paper system: We adopt servo motor control system which have features of high sensitivity, high speed and can ensure the body paper output smooth, no jam.

6. Adsorption system of body paper: We adopt the inverter control which cooperates with the solenoid valve`s speedy switch to ensure the body paper is adsorbed completely and the operation is simple.

7. Glue wheel: The glue wheel is made by steel with thickening electroplating and the surface is treated as fine grinding. The amount of glue can be adjusted to 8g/㎡ to ensure the glue is even for thick paper and thin paper.

8. Supplement of glue: We adopt automatic level control to ensure the supplement of glue is automatic and the production is continuous.

9. Paper separation: We adopt the disengaging pawl to its high speed movements and make the paper separate from the glue wheel effectively after being daubed glue.

10. Fit orientation: We adopt the prelocalization and both sides have their own side board to ensure the high speed fit precision.

11. Driving system: We adopt synchronous belt drive imported from the United States to ensure the transmission is accurate, steady and has low noise.

12. Operating system: The equipment takes the man-machine touch screen as a operation center which is contacted with PLC, so that kinds of information, such as warning, detective hitch, abnormal condition and operating speed can be displayed on the screen, it`s absolutely clear. This can also detect maintenance log book, logging and look over in real-time, dispose timely which can truly reflects the efficiency.

13. Electric system: We adopt domestic and international famous brand, design in accordance with the European CE standard, manufacture meticulously to ensure the overall unite is stable, has high efficiency and low hitch.

14. If the machine arrangement is changed without prior notice.

Glue Laminator Machine

Glue Laminator Machine, Gluing Laminator Machine, Fully Automatic Glue Laminator Machine

Anhui Innovo Bochen Machinery Manufacturing Co., Ltd. , https://www.innovomachines.com

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