The ABE (Acetone-Butanol-Ethanol) fermentation industry, characterized by the production of solvents such as acetone and butanol, was once the second largest fermentation industry in the world after ethanol fermentation. Among the three main products of ABE fermentation, butanol has the highest price, followed by acetone and ethanol. Therefore, fluctuations in butanol market prices have an important impact on the economics of ABE fermentation. In recent years, the price of petrochemical butanol has remained at a low level, making the economics of ABE fermentation difficult to compete with petrochemical butanol. In this case, improving the product value, increasing the conversion rate of raw materials, and using cheap raw materials are the basic ideas for improving the economics of ABE fermentation.
Researchers from the Li Yin research group of the Institute of Microbiology of the Chinese Academy of Sciences were inspired by Clostridium beijerinckii's ability to produce acetone, butanol, ethanol, and isopropanol from sugar, and designed and assembled an isopropanol bio The basic components of the synthesis module include the secondary alcohol dehydrogenase gene from C. beijerinckii and a strong promoter from C. acetobutylicum. This module was introduced into the high-butanol-resistant, high-yield solvent-producing mutant Rh8 (J. Proteome Res., 2010, 9: 3046-3061) selected in the early stage of the laboratory, and it was found that the acetone produced by the host cells can be efficiently and completely It is converted to isopropanol to create an isopropanol biosynthesis pathway, which transforms classic ABE fermentation into IBE (Isopropanol-Butanol-Ethanol) fermentation.
Isopropyl alcohol is an important chemical product and raw material. Currently, the world's total demand for isopropyl alcohol is about 2.3 million tons per year. The realization of Clostridium IBE fermentation can not only promote the realization of isopropanol biomanufacturing, but also mix isopropanol, butanol and ethanol also have the potential to be used as biofuel.
This research result has applied for a Chinese patent. The relevant paper was published online in Biotechnology for Biofuels on June 28. The first author is Dai Zongjie, a doctoral student jointly trained by the University of Science and Technology of China and the Institute of Microbiology, Chinese Academy of Sciences. The paper was published online less than one month ago, and has entered the top three of the journal Most viewed articles.
The research was supported by the 973 project's major scientific problem-oriented project "Synthetic Cell Factory" and the Chinese Academy of Sciences cooperative project.
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