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BioCommunications report: Drought stress seriously affects crop yields, and the losses caused by it are almost the sum of other natural disasters. Researchers from the College of Life Sciences of Henan University recently used the autonomous Mutator transposon maize material to hybridize with maize inbred line material to obtain the F1 insertion mutagenized population, F1 self-bred F2 population, and conducted in-depth mutant analysis Identification has provided experimental materials for further development of maize drought resistance breeding.
Drought stress severely affects the growth and development of corn seedlings and heading, especially in the early stages of corn growth, water deficit will greatly affect the biomass and the photosynthetic capacity of leaves, and ultimately inhibit the occurrence of reproductive organs and cause yield decline. Through proteomics research on two different varieties of maize under drought treatment, it was found that a large number of dehydration proteins, molecular partners and osmotic proteins are involved in the response to water deficit.
The corn Mutator (Mu) transposon has unique genetic characteristics such as high copy number, high frequency of forward mutations and a tendency to insert low-copy DNA sequences, and has become one of the important mutagenesis methods in genomics research Multiple genetic mutation systems, corn lines with autonomous Mutator, have a mutation phenotype of grains and seedlings that is 30 to 100 times higher than other corns. All the confirmed mutations are in the nuclear genome, the vast majority are recessive mutations, and dominant mutations are relatively rare.
In this article, the researchers used the autonomous Mutator transposon maize material 115F, 330I, 715D to cross with the maize inbred line material B73, Mo17, 97108, H9-21 to obtain F1 insertion mutagenic population, F1 self-bred F2 population. Observe the biological characteristics and phenotypic variation of agronomic traits of F2 individual plants in the field, and obtain mutants with different phenotypes. The mutants were statistically analyzed.
Under drought stress, the researchers used a far-infrared thermal imager to conduct a large-scale scanning of the leaf temperature of the F2 population seedlings planted in the indoor small bowl, and selected plants with obvious leaf temperature differences as potential mutants. More than 38,000 plants, 108 drought-resistant single plants and 121 drought-sensitive single plants were successfully screened. Repeated infrared temperature detection, chlorophyll fluorescence analysis, leaf water loss, photosynthetic characteristics analysis, and determination of soluble sugars, soluble proteins, and proline content The mutants were initially identified and analyzed. MuTAIL-PCR was used to clone the obtained mutants. The research provided experimental materials for further development of maize drought resistance breeding
Recently, due to the development of maize genome sequencing and related bioinformatics, it may become a new model plant to reveal the molecular genetic mechanism of its response to the outside world and the biological basis for the formation of important agronomic traits. The selection of related mutants is very necessary and urgent.
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