Today, Affymetrix's Panomics brings branched-chain DNA technology to scientific research to directly quantify mRNA, miRNA, ncRNA, fusion transcripts, and DNA copy number variation. No RNA extraction, no reverse transcription, no PCR, only by hybridization with the probe and signal amplification, you can perform gene quantification, and can quantify up to 80 genes in a single reaction. In addition, it is also very simple in operation and data processing. All probes are designed and synthesized by the manufacturer, you only need to prepare samples and wait for the experiment. All experimental data are all linearized results, and there is no need to perform the corresponding â–³ Ct conversion. So you don't have to worry about the complicated data processing after the experiment.
QuantiGene analysis includes single and multiple analysis forms. Single gene detection does not require expensive equipment, only ordinary chemiluminescence detector to detect readings. Multigene detection can be operated in conjunction with the Luminex system. This analysis is suitable for a variety of samples, and ordinary cell lysates are of course. Even challenging FFPE samples can be easily handled without encountering the common inhibition, false positives and false negatives in PCR quantification problem.
QuantiGene® 2.0 Assay enables sensitive and direct determination of miRNA, RNA or DNA copy number in any sample. This simple hybrid analysis uses a process similar to ELISA to generate glow signals, which can be detected using a standard 96-well luminescence detector.
QuantiGene® 2.0 Assay is widely used to quantify gene expression in signal transduction pathways, as well as retrospective and prospective biomarker validation of FFPE samples. They are ideal for applications such as quantification of miRNA and fusion transcripts, and detection of a small number of biomarkers (from 1 to no more than 100) in a large number of samples.
Features and advantages
• Diversity – direct quantification of multiple difficult sample types, including FFPE and fresh frozen tissue, blood, urine, cells, miRNA, RNA, DNA, etc.
• Simplified – removes unnecessary steps and biases related to miRNA, RNA or DNA purification methods, cDNA synthesis and PCR amplification
• Ignore degradation samples – insensitive to base chemical modification and RNA degradation due to formalin fixation
• High specificity – use multiple probes per target to generate signals based on cooperative hybridization events
QuantiGene® Plex Assay-multiplex analysis of RNA and DNA
QuantiGene® Plex Assay is a cost-effective method for producing highly reliable, high-quality, and repeatable results from multiple targets in large numbers of samples. Identifying and evaluating gene expression characteristics allows researchers to track multiple genes related to specificity, potency, and toxicity, thereby identifying targets and optimizing lead compounds.
QuantiGene® Plex 2.0 RNA Assay and QuantiGene® Plex DNA Assay are widely used in the analysis and quantification of genetic characteristics, suitable for the verification of biomarkers in retrospective and prospective studies (using FFPE tissue and blood samples), chip and sequencing results , Predictive toxicology, DNA copy number analysis and others.
Features and advantages
• Efficient – ​​multiplex analysis of up to 80 RNA targets (RNA) or 34 DNA targets (DNA) in the same well, saving time and cost
• No intermediate steps – removes steps for RNA and DNA purification, cDNA synthesis and PCR amplification
• Reliable-Insensitive to chemical modification of base and RNA degradation due to formalin fixation
• Low starting volume – usually only 10 ml of blood is required for each analysis
• High specificity – multiple probes per target, based on cooperative hybridization events
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