Objective: To study the status of rabbit bone marrow mesenchymal stem cells (MSCs) induced to differentiate into endothelial progenitor cells (EPC) in vitro.
Methods: Rabbit bone marrow bone marrow was isolated, and bone marrow mesenchymal stem cells were isolated and cultured by density gradient centrifugation. The cells of the experimental group were differentiated into endothelial progenitors under the induction conditions of endothelial cell growth additives, vascular endothelial growth factor, fibroblast growth factor, etc. cell. The control group continued training without intervention.
Results: When the colonies of the experimental group were close to confluence, the morphology showed the appearance of "cobblestone" or "paving stone". Flow cytometry showed that the cells in the experimental group expressed CD133 and CD34 more than the control group (P <0.05). Conclusion: In vitro induction can induce bone marrow mesenchymal stem cells to differentiate into endothelial progenitor cells and effectively expand.
1 Materials and methods Ll General information 3 to 5 month old Japanese big-eared white rabbits 10 (provided by the Laboratory Animal Center of Wuhan University School of Medicine), weighing 2 to 2.5 kg, male or female. Reagents are: special grade fetal bovine serum, DMEM medium, Pereoll separation solution, endothelial cell additive (ECGS), vascular endothelial growth factor basic fibroblast growth factor, trypsin, CD133, CD24 monoclonal antibodies
Studies have reported that stem cell transplantation represented by endothelial precursor cells can promote angiogenesis and improve myocardial perfusion. It has broad prospects for the treatment of coronary heart disease, but the endothelial progenitor cells (EPC) content in stem cells is extremely low, which affects its efficacy to a certain extent. This study aims to conduct basic research for EPC transplantation by observing the differentiation and expansion of mesenchymal stem cells (MSC) into endothelial progenitor cells (EPC) under appropriate induction conditions.
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