Chromatin DNA digestion analysis

Chromatin DNA digestion analysis

The bacterial nucleus is composed of a circular chromosome without protein or histone. It is essentially a naked double-stranded DNA. The genes on it are different from eukaryotic cells. They are continuous and contain no introns. The mRNA can be directly translated into polypeptide without cutting and splicing.

Bacterial chromatin DNA, like plasmid DNA, can also be digested with restriction enzymes. The resulting DNA fragments can be separated by agarose gel electrophoresis. The electrophoretic pattern formed by each genome is also specific. endonuclease analysis (restriction endonuclease analysis) pattern technology, but the obtained pattern depends on the type of endonuclease used, you can get a different electrophoresis pattern, in order to facilitate visual observation, you can apply large fragments, less band Enzyme digestion is better, and then use the labeled gene probe for detection.

REA map technology can be used to identify bacterial species, even different strains of the same kind of bacteria. If all members of a genus or a species have the same REA map, it cannot identify bacteria.