English name: TRIS-HC1; Tri (hydroxymethyl) aminomethane hydrochloride; 2-Amino-2- (hydroxymethyl) -1,3-propanediol hydrochloride; Tromethane hydrochloride; Trizma hydrochloride
Other names: tris (hydroxymethyl) aminomethane hydrochloride; 2-amino-2-hydroxymethyl-1,3-propanediol hydrochloride
CAS number: 1185-53-1
C4H11NO3 · HCl = 157.60
Level: BR
Content: ≥99.0%
PH (1% solution H20, 25 ℃): 4.2 ～ 5.0
Loss on drying: ≤0.5%
Melting point: 150 ～ 155 ℃
Absorbance (260nm): ≤0.06
Properties: white crystal or crystalline powder. Soluble in water, pKa (25 ° C) 8.1
Uses: biochemical research. Biological buffer, organic synthesis intermediate. The pH of all buffers depends on temperature and concentration. For Tris buffer, for every 1 ° C increase in temperature, the pH increases by approximately 0.03 units, and for every 10-fold dilution the pH decreases by 0.03-0.05 units.
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